Determination of Arsenic Species in Beer Samples by Liquid Chromatography-Hydride Generation Atomic Fluorescence Spectrometry

Based on the hydride generation—atomic fluorescence spectrometry detection and liquid chromatography separation,an analytical method for arsenic speciation in beers was developed.Four arsenic species,i.e.,arsenite As(Ⅲ), dimethylarsinic acid(DMA),monomethylarsonic acid(MMA) and arsenate As(Ⅴ) were separated by anion-exchange chromatography with isocratic elution using 20 mmol/L KH2PO4/K2HPO4 as the mobile phase with the flow rate of 1 mL/min.The elution time were 2.80,3.90,5.42 and 12.01 min for As(Ⅲ),DMA,MMA and As(Ⅴ),respectively.Potassium borohydride(3.5%,m/V) and hydrochloric acid(10%,m/V) were used as the reduction agents and the argon with a flow rate of 500 mL/min was used as the carrier gas to deliver the volatiled species.The detection limits of the As(Ⅲ),DMA,MMA and As(Ⅴ) were 0.8 μg/L,1.6 μg/L,0.8 μg/L,2.1 μg/L,respectively.The linear response for the four arsenic species were obtained in the range of the 0~200 μg/L,and the recovery values were between 91.4%~104.4%.The developed method is simple,rapid and sensitive,and suitable for arsenic speication in beer sample.