Study on antibiotics resistance and outer membrane channel protein OprD_2 of Pseudomonas aeruginosa

Objective To investigate distribution and drug resistance of Pseudomonas aeruginosa(PA), and analyse the relationship between deletion of outer membrane channel protein OprD2 in PA and its imipenem resistance. Methods The anti-microbial susceptibility test was performed by K-B slip diffusion method in all 949 Pseudomonas aeruginosa isolates from 2011 to 2013. Polymerase chain reaction(PCR) was used to amplify oprD2gene. Results In the 949 Pseudomonas aeruginosa strains, the detection rates of PA in neurosurgery and ICU were the top two highest, accounted for 25.40% and 22.76%, respectively. Of the antibiotics sensitive rate to Amikacin was the highest(85.88%),the second was Aztreonam(85.25%) and the highest resistant rate was Ceftazidime(37.09%).312 of 949 strains were detected resistant Imipenem,the resistant rate of IRPA was 32.88% and 42 strains were lack of oprD2gene, with a loss rate of 51.85%.Conclusions The drug resistance phenomenon of PA is serious. The loss of OprD2 may contribute to the resistance of PA to Imipenem.