Optimization of the Extraction Technology of Lycium barbarum Polysaccharide by Response Surface Methodology

Objective To optimize the extraction technology of Lycium barbarum polysaccharides by response surface methodoloy.Methods Experiment factors and levels were firstly selected by one factor tests.On the basis of the previous results and according to the central composite experimental design principles,the method of Response Surface Method with 3 factors and 3 levels was adopted.Then the factors influencing the technological parameters were determined by means of regression analysis.Response surface and contour were finally graphed with the extraction rate as the response value.Results The optimum conditions of Lycium barbarum polysaccharides extraction were as follows:the ratio of material to solvent of 1∶32,extraction temperature of 84℃ and extraction time of 2.3 h,one time extraction.Conclusion The yield of Lycium barbarum polysaccharides is 11.10% under the optimum conditions.