2004•Zhonghua shiyan waike zazhiRequires access
Change of tissue and blood protoporphyrin IX in rat colorectal carcinoma
Yang Zhang
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Abstract
Objective To investigate the changes in levels of blood and tissue protoporphyrin IX in Sprague Dawley rats with colorectal carcinoma and normal Sprague Dawley rats.Methods Seventy Sprague Dawley rats were divided into two groups:experimental group ( n = 60) and control group ( n = 10). In experimental group,DMH (25 mg per kg) was injected into rat abdominal cavity once a week for 18 weeks.In control group,rats were not treated by DMH.At 20th week blood protoporphyrin IX in two groups and tissue protoporphyrin IX in experimental group were examined by fluorospectrophotometer.Results Blood protoporphyrin IX in experimental group and control group was (2.224± 0.323) mg/L, (1.663± 0.092) mg/L respectively.Blood protoporphyrin IX in experimental group was higher than that in control group ( P 0.01). Blood protoporphyrin IX/Hb in experimental group and control group was (25.908± 12.540) μg/g and 10.609± 0.929 μg/g respectively.Blood protoporphyrin IX/Hb in experimental group was higher than that in control group ( P 0.05). Protoporphyrin IX in normal tissue,early stage colorectal carcinoma and advanced colorectal carcinoma was (317.099± 16.859) ng/g, (416.814± 6.786) ng/g and (606.874± 21.798) ng/g respectively.The protoporphyrin IX in advanced colorectal carcinoma was higher than that in normal tissue,early stage colorectal carcinoma ( P 0.01). The protoporphyrin IX in early stage colorectal carcinoma was higher than that in normal tissue ( P 0.01). Conclusion Blood and tissue protoporphyrin IX levels are increased in colorectal carcinoma.Blood protoporphyrin IX may be used as a factor to screen colorectal carcinoma.