Researches on quantitative analysis of accumulation of protoporphysin IX in colorectal cancer cells induced by drugs

【Objectives】The quantitative analysis on the characteristics of accumulation of the protoporphysin Ⅸ of normal tissue,earlier carcinoma tissue,progression carcinoma tissue of colorectal cancer of rat induced by various drugs. 【Methods】15 SD rats with colorectal cancer were divided into three groups. Group A were injected with 5ALA through vena caudalis. Group B were injected by DFO and 5-ALA,first which was injected with DFO through intraperitoneal,after a half hour,injected with 5-ALA through vena caudalis. Group C were injected with isotonic Nacl chloride through vena caudalis. Samples of each group have been gathered after having been induced for 4 hr. Samples of the other 20 rats have been gathered respectively at 2,4,6,8,10 h induced by DFO + 5-ALA. The content of protoporphysin Ⅸ was detected by high performance liquid chromatogram and fluorescence detection. 【Results】5-ALA induced group,the content of protoporphysin Ⅸ of normal tissue,earlier colorectal cancer tissue,progression colorectal cancer tissue respectively acted:(475.261±65.027),(687.738±73.293),(852.371±69.457) ng/ mg; DFO and 5-ALA combine-induced group respectively acted:(496.194±63.539),(853.194±81.937),(1173.795± 87.184) ng/mg. Group C respectively acted:(347.375±57.413),(479.375±59.274),(593.173±37.461) ng/mg. The content of protoporphysin IX of the carcinom tissue achieved tiptop after having been induced for 4 h,which of the normal tissue achieved tiptop after having been induced for 2 hr,which of carcinoma tissue and normal tissue has the most difference after having been induced for 4 hr. 【Conclusions】5-ALA combined with DFO significantly increased the accumulation of protoporphysin Ⅸ in 5-ALA-induced carcinoma. The content of protoporphysin Ⅸ achieved maximum after having been induced by drugs for 4-6 h.