2005•Zhōnghuá yàoxué zázhìRequires access
Study on tissue distribution difference of cytochrome P450 2C19 in Chinese population Han
Wu Minliang, Xiaohong Wu, Shuqing Chen
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Abstract
OBJECTIVE To detect CYP2C19 mRNA and CYP2C19 enzyme protein expression in 11 normal tissues,including liver tissue,in Chinese population Han. METHODS CYP2C19 mRNA expression level was detected in 11 normal tissues, including liver, colon, stomach, breast, esophagus, lung, uterus, brain, pancreas, ovary, kidney. Several hepatic carcinoma cell lines, colorectal cancer cell lines and uterus cancer cell line were determined by semi-quantitative RT-PCR.CYP2C19 enzyme protein was detected by qualitative western blotting technology. RESULTS The results showed that CYP2C19 mRNA only expressed in liver tissue and hepatic carcinoma cell lines, CYP2C19 enzyme protein was found in normal liver tissue, hepatocarcinoma cell lines HepG2, HepGA and 7721. The result was in accordance with the result of RT-PCR based on tissue location.CONCLUSION The investigation of tissue distribution of CYP2C19 mRNA and CYP2C19 enzyme protein in Chinese population Han will lead us to know the drug metabolization through CYP2C19 in different tissues.