The effect of bone marrow mesenchymal stem cell transplantation on cerebral infarction in rats

Objective To observe the effect of transplanted bone marrow mesenchymal stem cells on the brain of rats with cerebral infarction.Methods Fifty middle cerebral artery occlusion model were randomly divided into control group (n = 6),group with supernatant transplantation(n = 22) and group with BMSCs transplantation(n =22).3h after animal model was established,PBS,supernatant and BMSCs was injected intravenously in three groups respectively.28d after transplantation, the survival condition of transplanted bone marrow mesenchymal stem cells in the brain of rats with cerebral infarction were observed,the expression of neuron specific enolase in brain tissue and the neurological severity score(NSS) were compared among all the groups 0,7,14 and 28d after operation.Results At day 28 after bone marrow mesenchymal stem cell transplantation,under fluorescence microscope there were a large quantity of BrdU positive cells aggregated in the marginal zone of cerebral infarction focus(hippocampus),while the quantity of BrdU positive cells in the uninjured side of brain tissue was less.A small quantity of BrdU positive cells in the marginalzone of cerebral infarction focus expressed neuron specific enolase. The NSS of the rats with BMSCs transplantation was lower than supernatant group and PBS group(P0.05),while no significant difference was found between the later two groups(P0.05 ).Conclusion After transplanted bone marrow esenchymal stem cell into middle cerebral artery occlusion rats through vein,bone marrow mesenchymal stem cells can survive and migrate to the peri-infarct region,and transfered into neuron like cell,which has a better effects.