Protect Effect of Combinded Therapy of Herbal and Benazepril on Early Stage of Diabetic Nephropathy in Rats

Objective:To study the effect and mechanism of combined Chinese and Western medicines in early diabetic nephropathy(DN) in rats.Method:The male wistar rats were randomly divided into 2 groups:normal and DN groups.Experimental DN was induced in DN group with streptozotocin(STZ),and the normal group was given equal volume of distilled water.Then the modeled rats were subrandominzed into model group and benazepril(5 mg·kg-1)+herbal group(50 g·kg-1).Benazepril+herbal group was medicated respectly,the other two groups were given distilled water.After the rats had been treated for 8 weeks,transforming growth factor-β1(TGF-β1),collagen Ⅳ,angiotensinⅡ(AngⅡ),monocyte/macrophage(ED-1) in the renal tissues of the rats were detected by immunohistocytochemistry.Result:Compared with model group,the thickened glomerular basement membrane,increased mesangial cells,proliferated mesangialmatrix,and enlarged mesangial area became lightened in benazepril+herbal group after treatment.Moreover,TGF-β1,collagen Ⅳ,AngⅡ and ED-1 were reduced when compare to model group,but which were higher than normal group.Conclusion:This recipe combined with benazepril can reduce the pathological damage of early DN and postphone the development of DN.