Expression of tumor suppressor candidate gene 4 and Raf kinase inhibitor protein in different grade of ovarian serous carcinoma

Objective To evaluate the prognosis and guide clinical treatment by studying expression of tumor suppressor candidate gene 4(TUSC4) and Raf kinase inhibitor protein(RKIP) in different grade of ovarian serous carcinoma(OSC).Methods Seventy-seven cases of OSC specimens which were given resection and diagnosed by pathological analysis were divided into ovarian junior serous carcinoma group(25 cases),ovarian senior serous carcinoma group(52 cases);53 cases ovarian serous cystadenoma which were given resection and diagnosed by pathological analysis and 23 cases ovarian borderline serous cystadenoma were also enrolled in the study;25 cases normal oviduct tissue which were given resection for other reasons and 25 cases normal ovarian epithelial tissues were chosen as control. Using immunohistochemical method to detected the expression of TUSC4 and RKIP in the six groups,and the positive expression rates of TUSC4 and RKIP were compared and analyzed in the six groups. Results The positive expression rate of TUSC4 of normal ovarian epithelial tissues,ovarian serous cystadenoma,ovarian borderline serous cystadenoma,ovarian junior serous carcinoma,ovarian senior serous carcinoma and normal oviduct tissues was 80. 0%,73. 6%,60. 9%,48. 0%,32. 7%,32. 0%,respectively. The positive expression rate of TUSC4 in ovarian junior serous carcinoma was lower than that in ovarian serous cystadenoma(χ2= 4. 91,P 0. 05) and normal ovarian epithelial tissues(χ2= 5. 56,P 0. 05). The positive expression rate of TUSC4 in normal uterine tubal epithelium tissue and ovarian senior serous carcinoma was lower than that in Ovary borderline serous cystadenoma(χ2= 4. 02,5. 22;P 0. 05),ovarian serous cystadenoma(χ2= 12. 27,17. 63;P 0. 01)and normal ovarian epithelial tissue(χ2= 11. 69,15. 14;P 0. 01). The positive expression rate of RKIP of normal ovarian epithelial tissues,ovarian serous cystadenoma,ovarian borderline serous cystadenoma,ovarian junior serous carcinoma,normal oviduct tissues and ovarian senior serous carcinoma was 100. 0%,84. 9%,69. 6%,64. 0%,52. 0%,13. 5%,respectively. The positive expression rate of RKIP in ovarian senior serous carcinoma was lower than that in normal ovarian epithelial tissue,ovarian serous cystadenoma,ovary borderline serous cystadenoma,ovarian junior serous carcinoma and normal uterine tubal epithelium tissue(χ2= 52. 06,53. 59,23. 61,20. 59,13. 04;P 0. 01). The positive expression rate of RKIP in ovary borderline serous cystadenoma was lower than that in normal ovarian epithelial tissue(χ2= 8. 91,P 0. 01). The positive expression rate of RKIP in normal uterine tubal epithelium tissue and ovarian junior serous carcinoma lower than that in normal ovarian epithelial tissue(χ2= 15. 79,10. 98;P 0. 01) and ovarian serous cystadenoma(χ2= 9. 65,4. 36;P 0. 05). Conclusions TUSC4 and RKIP are all involved in the pathogenesis of ovarian junior serous carcinoma,RKIP is also involved in the pathogenesis of ovarian senior serous carcinoma. The pathogenesis of ovarian junior and senior serous carcinoma may be different.