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2002•Unpublished venueRequires access

Subcloning of GNA mature protein (MGNA) gene and its construction of expression vector in E.coli

Luo Su

1 citations

Abstract

Snowdrop lectin mature protein is toxic to sap sucking insects and some chewing pests and many kinds of nematode.Mature protein gene of snowdrop lectin (MGNA) was subcloned in T vector from plasmid containing its precursor protein gene.MGNA was inserted downstream of T7 promoter at three different sites of E.coli expression vector pET22b(+),resulting in the construction of three prokaryotic expression vectors p22bG1(secretion of fusion GNA),p22bG2(Inclusion body) and p22bG3 (secretion of native GNA). The three vectors give different types of MGNA product expressed in E.coli. This research will be the base for MGNA expression in E.coli and make it into bio-pesticide.

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What this paper is about

Snowdrop lectin mature protein is toxic to sap sucking insects and some chewing pests and many kinds of nematode.Mature protein gene of snowdrop lectin (MGNA) was subcloned in T vector from plasmid containing its precursor protein gene.MGNA was inserted downstream of T7 promoter at three different sites of E.coli expression vector pET22b(+),resulting in the construction of three prokaryotic expression vectors p22bG1(secretion of fusion GNA),p22bG2(Inclusion body) and p22bG3 (secretion of native GNA). The three vectors give different types of MGNA product expressed in E.coli. This research will be the base for MGNA expression in E.coli and make it into bio-pesticide.

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Available abstract

Snowdrop lectin mature protein is toxic to sap sucking insects and some chewing pests and many kinds of nematode.Mature protein gene of snowdrop lectin (MGNA) was subcloned in T vector from plasmid containing its precursor protein gene.MGNA was inserted downstream of T7 promoter at three different sites of E.coli expression vector pET22b(+),resulting in the construction of three prokaryotic expression vectors p22bG1(secretion of fusion GNA),p22bG2(Inclusion body) and p22bG3 (secretion of native GNA). The three vectors give different types of MGNA product expressed in E.coli. This research will be the base for MGNA expression in E.coli and make it into bio-pesticide.

Key concepts: Subcloning, Biology, Expression vector, Vector (molecular biology), Gene, Plasmid, Gene expression, Molecular biology

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Subcloning of GNA mature protein (MGNA) gene and its construction of expression vector in E.coli — Research Paper | ScholarLens