The effects of hepatic stimulator substance on homing of bone marrow mononuclear cells to liver

Objective:To explore the effects of hepatic stimulator substance on homing to the liver of bone marrow mononuclear cells in mice.Methods:Mouse hepatic injury was induced by intraperitoned injection of carbon tetrachloride(CCl_(4)) and adnunistration of 2-acetylminofluorene(2-AAF).Bone marrow stem cells seperated from donor mice were labeled with PKH26 and then were transplanted through tail vein to recipient mice whose liver were injured.HSS was injected intraperitoneal in 80 μg/(kg·d) to the experiment group,and glucose was injected to the control group.Two week after transplantation,mice liver were taken out and PKH26 labeled cells were counted in frozen section.Then histological assessment was measured in H-E stained section.Results:The average of PKH26 labeled positive cells in frozen sections in HSS group were more than that in the control group,84 vs 61(P﹤0.05).Regenerative hepatocyte in HSS group were more than that in the control group.Conclusion:Hepatic stimulator substance promotes homing of bone marrow mononuclear cells to the liver.