The preparation and identification of anti-HBV G145R HBsAg polyclonal antibody

Objective To prepate the mouse polyclonal Anti G145R rHBsAb and analyze its immunological characteristics.Methods The recombinant G145R mutant HBsAg were respectively used to develop poly-clonal antibodys through immunize BALB/c rats,and the polyclonal antibody were confirmed by empirical methods,include western blot,ELISA and SDS PAGE,and preliminary used in chemical staining for the CHO.Results The recombinant G145R mutant HbsAb poly were indentified by western blot and ELISA,it showed a good specific reaction performence to both of G145R rHBsAg and r-wHBsAg.And the potency of the reaction with G145R rHBsAg was obviously higher than r-wHBsAg(they were 51200 and 6400).And it had a good result of the PAP staining of cell 2A8 also.Conclusion The satisfactory antibody could be created by immunizing with G145R rHBsAg.Because of the cross reaction with r-wHBsAg,the recombinant G145R mutant HbsAb poly which is mixed with r-wHBsAg can enhance the detectivity of ELISA.