2012•Xiandai shengwu yixue jinzhanRequires access
A Comparison Analysis between the Dynamic Expressions of Serum miR-21 and AFP During the Process of Liver Cancer Development in Rats
Dou Ke-feng
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Abstract
Objective: To investigate whether serum microRNA-21(miR-21) could be used as a novel biomarker of liver cancer by comparing with serum AFP.Methods: Diethylnirtosamine(DENA) abdominal injection was applied to induce liver cancer in F344 rats.Blood samples of rats were collected for analysis.Real-time quantitative PCR technique and ELISA test were used respectively to measure the expressions of serum miR-21 and AFP during the liver cancer developing process.Results: Comparing with that in normal rats and liver fibrosis rats,miR-21 had higher expressions in liver cirrhosis rats,early liver cancer rats and advanced liver cancer rats(P0.05),while AFP had higher expressions only in early and advanced liver cancer rats(P0.05).During the developmental process of liver cancer,the miR-21 expression kept on increasing,while the AFP expression only increased at the stage of tumor formation.Conclusion: Data indicated that MiR-21 played an important role in liver cancer development and had diagnostic value for indicating early liver cancer.