2010•Zhongguo yaofangRequires access

Determination of Total Flavonoids from Girardinia diversifolia by UV Spectrophotometry

Gao Yu-qiong

Abstract

OBJECTIVE:To establish the determination method for total flavonoids from Girardinia diversifolia.METHODS:The contents of total flavonoids were determined by UV spectrophotometry at detection wavelength of 268 nm with apigenin as standard substance.RESULTS:The linear range of apigenin were 1.984～11.904μg·mL -1(r=0.999 9)with average recovery of 96.15%(RSD=1.98%,n=6).CONCLUSION:The method is accurate,reliable and stable and it can be used for the content determination of total flavonoids from G.diversifolia.

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Key concepts: Apigenin, Spectrophotometry, Chemistry, Chromatography, Linear relationship, Flavonoid, Mathematics, Biochemistry