Flow cytometry analysis of actue leukemia immunophenotyping

Objective To analyse actue leukemia immunophenotyping by flow cytometry and provide valuable information for clinical treatment and diagnoses.Methods Apply two color immunofluorescece antibody marking single cell of patients' oute blood or marrow,analyse immunophenotyping of these marked leucocyte by flow cytometry.Results 76 actue leukemia cases were analysed,12 cades were T-ALL occuping 15.8%,22 cases were B-ALL occuping 28.9%,36 cases were AML occuping 47.4% and 6 cases were HALM/L.Occuping 7.9%.Patients with ALL expressed both lymphocyte antigen and myeloid antigen.Patients with AML expressed myeloid antigen,a small part cases expressed lymphocyte antigen at the same time.Patients with HAML/L all expressed lymphocyte antigen and cmeloid antigen.Expression rate of AML' cell antigen in adult is remarkably higher than that in children(75%/27%).Conclusion Flow cytometry can analysis and distinguish all types of actue leukemia excatly,it is an important method for leukemia' diagnoses and type defining.