Characteristic analysis of actue myeloid leukemia immunophenotyping by using four-color flow cytometry

Objective To study the characteristic of immunophenotype of actue myeloid leukemia(AML) and its significance.Methods Four-color direct immunofluorescence staining methods of flow cytometry and CD45/SSC gating analytical technique were taken for detection of 105 cases of AML,and antibody integration system were used for immunophenotype.Results Immunophenotyping of 105 cases of AML showed that all antigens expressed positive were CD13(97.1%),CD33(90.5%),MPO(79.0%)by turns and stem/progenitor cells differentiation antigen expressed were CD117(77.1%),human leukocyte antigen locus DR(HLA-DR)(72.4%),CD34(54.3%) by turns.Patients with AML accompanied positive lymphocyte antigen expressed CD19,CD7,CD2,CD10,CD8.CD34+CD11b+ and CD34+CD11c+ were the two most common expressed abnormality of bestride the stage.CD13,CD33 and HLA-DR were the most visible in the antigen deletion expressed.Conclusion Four-color direct immunofluorescence staining methods of flow cytometry has important significance for special types of AML,and it can help to guide clinical diagnosis,treatment and prognosis.