Construction of schistosoma japonicum (chinese mainland strain) egg full length cDNA library

Mature egg is the primary agent of pathogenesis in hosts and is responsible for dissemination of the parasite.Therefore,todevelop anti-eggimmunityis significant for preventing schistosomiasis and blocking transmis-sion.Egg full length cDNA library of Schistosoma japonicum was constructed by using SMART cDNA library con-struction kit.The capacity of this egg library was 7.55×106,in which more than 97% clones are recombinant.Three known full length gene: SjPP,SjTEGT and SjeIF 5 were successfully amplified from egg cDNA library.Furthermore,a newfull length gene,Enolase,was also successully fished.These datas showthat egg full length cDNA library is of resonably good quality,and can be serve as the valuable material for screening the protective antigen genes,particu-larlyanti-eggantigen genes.