17β-ESTRADIOL INHIBITED ACUTE DAMAGE OF LIVE FUNCTION BY ELEVATING HEPATOCYTE GROWTH FACTOR

Objective: To explore the significance of expression of hepatocyte growth factor(HGF) in 17β-estradiol(E2) inhibiting acute damage of liver function.Methods:40 male rats were divided into 4 groups, including group A、B、C and D. Acute hepatocyte injury was induced in all male rats by CCL4 administration about 4 weeks. In addition, rats in group A were treated with HGF(100 mg/(kg·d)), rats in group B were treated with HGF(100 mg/(kg·d)), rats in group C were control group, and rats in group D were treated with tamoxifen(6 mg/(kg·d)). Then we collected the portal vein blood and liver tissue from every rat at the end of 4th week for our research working. We detected the serum quantities of alanine aminotransferase(ALT) and serum total bilirubin(TBIL) by biochemical analysator, detected the expressions of HGF and PCNA in all rat liver by immuno- histochemistry(SP),detected the expression of HGFmRNA in rat livers by Real-Time PCR(SYBR GREEN 1).Results:(1) The quantities of serum ALT and TBIL in all rat groups were increasing progressively respectively as: ABCD, the comparisons of above four groups were statistically significant(F(ALT)=407.410,F(TBIL)=144.872,P0.05). (2) The expressions of HGF in all rat groups were decreasing progressively as: ABCD, and comparisons of above four groups were statistically significant(F=5.148,P0.05); The expressions of PCNA in all rat groups were decreasing progressively as: ABCD, and comparisons of above four groups were statistically significant(F=5.627,P0.05). (3) The expressions of HGFmRNA in all rat groups were decreasing progressively as: ABCD, and the comparisons of above four groups were statistically significant(F=32.537,P0.05). (4) In all rat liver tissues, HGF and PCNA were positively linearly correlated (r=0.370,P0.05). Conclusion: E2 can enhance proliferations of hepatocyte to improve hepatic function by elevating the expressions of HGF in rat liver tissue, which can inhibit CCL4 inducing Wistar rat acute damage of liver function.