2005•Chinese Journal of Integrated Traditional and Western NephrologyRequires access
Protective Effect of Shenkangpian On Adriamycin Nephrotic Rats
Qi Qi
Open publisher page 0 citations
Abstract
Objective:To investigate the protective effect of Shenkangpian on adriamycin(ADR) nephrotic rats and the corresponding mechanism.Methods:Nephrotic syndrome was induced by intravenous injection of ADR. Rats were randomly divided into 5 groups:model control group,Shenkangpian high-dosage group ,Shenkangpian low-dosage group, Benazapril group and normal control group. Quantity of 24h urinary protein excretion, albumin, blood cholesterol,triglyceride,serum creatinine,BUN and malondialdehyde(MDA), saproxide dismatase (SOD) and pathological changes of kidney were examined after 6 weeks. Immunohistochemistry was used to examine the expression of fibronectin(FN), laminin(LN): Collagen Ⅳ, transforming growth factor β 1(TGF-β 1) and interleukin one (IL-1) in kidney tissues.Results:The excretion of urinary protein and the triglyceride were significantlly reduced by Shenkangpian after 6 weeks treatment as compared with model group.Immunohistochemistry analysis showed that the expression of FN,LN, collagen Ⅳ, TGF-β 1 and IL-1 in the kidneys were decreased by shenkangpian. MDA in kidney homogenates was decreased,while SOD was increased notably as compared with model group.Conclusion:Shenkangpian can relieve renal leisions of adriamycin nephropathy by decreasing extracellular matrix accumulation, inhabiting TGF-β 1 and IL-1 expression and reducing oxygen free radicals in kidney of rats.