2003•Zhonghua shiyan waike zazhiRequires access
The influence of colchicine on glucose uptake by C6 glioma cells
LI Fang-cheng
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Abstract
Objective To observe the influence of colchicine on glucose uptake by C6 glioma cells. Methods C6 glioma cells were incubated with colchicine for 3 h at 37℃, and then the cells were incubated in 1 ml PBS containing 125μmol/L unlabeled 2-deoxy-D-glucose and 37 × 103 Bq [3H]2-de-oxy-D-glucose for 5 min at 37℃. By using liquid scintillation analyzer, the glucose uptake by C6 glioma cells was determined. Results Colchicine could inhibit basal glucose uptake in a concentration-dependent manner by 7 % to 27 % . Glucose uptake was increased with carbachol by 11 % to 24 % , but this action was abolished by pretreatment with colchicines. Basal glucose uptake was increased by insulin in a concentration-dependent manner by 35 % to 47 %, but this action was not affected by pretreatment with colchicines. Conclusion Colchicine is involved in basal and carbachol-stimulated glucose uptake by C6 cells.