The relations between growth rate of glioma cell and glucose uptake

Objective To explore the relations between growth rate of glioma cell and glucose transport system.Methods C6 glioma cells were subcloned to produce C6 1,C6 2,C6 3 and C6 4.The level of GLUT1 mRNA and their protein of C6 1 cells and C6 4 cells were measured by Northern blot and Western blot respectively;at the same time,The rates of uptake of glucose by C6 4 cells and C6 1 cells were measured.Results The growth rates of the four cell clones differed from each other.The growth of C6 4 cells was about eight times faster than that of C6 1 cells.These clones predominantly expressed the GLUT1,the level of GLUT1 mRNA was markedly increased in C6 4 over C6 1 cells,densitometric analysis of the bands showed that the amount of GLUT1 protein in C6 4 cells was about three times higher than that in C6 1 cells [(310±21)%, P 0.001].The rates of uptake of 3 O methylglucose assayed by liquid scintillation count per minute:C64 cells was (7 680±180)/min·10 4cells,C6 1 cells was (3 000±125)/min·10 4cells;C6 4 cells was about 2.5 times higher than that by C6 1 cells.Conclusion These data suggest that the glucose transport system,coupled with glucose uptake,is closely related to the growth of glioma cells.