Change in the expression of interleukin-6 and tumor necrosis factor-α mRNA in frontal lobe and in protein level in serum in diffused traumatic brain injury rat

Objective:To investigate the change in the expression of interleIukin-6(IL-6) and tumor necrosis factor-α(TNF-α)mRNA in frontal lobe and serum IL-6 and TNF-α level in diffused traumatic brain injury rat model.Methods:Mamarou rat model of diffused traumatic brain injury was used in the study.Venous blood and frontal lobe were collected at different time after injury.The change of IL-6 and TNF-α mRNA expression and serum IL-6 and TNF-α level were detected with RT-PCR method and radioimmunoassay,respectively.Results:it was found that IL-6 mRNA level increased in diffused traumatic brain injury compared to the control group and reached to the peak 6 hours after injury.Serum IL-6 level also elevated in diffused traumatic injury rat compared to the control group and reached to the peak 12 hours after injury.TNF-α mRNA expression was up-regulated compared to the control group and reached to the peak 12 hours after injury.Serum TNF-α level elevated gradually within 24 hours after injury.Conclusion:Diffused traumatic brain injury induces upregulation of IL-6 and TNF-α mRNA expression in frontal lobe and elevation of serum level of them.IL-6 and TNF-αmay play an important role in the progress of diffused traumatic brain injury.