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Determination of TPS by Improvement of Anthrone-sulfuric Acid Method

Wenshui Xia

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Abstract

The procedure of colorimetric analysis of TPS was investigated in this paper. The results showed that the accuracyof determination was largely improved by processing with the following procedure: 4ml of 0.05% anthrone in concentrated sulfuricacid was added to 1ml TPS solution and heated 5min at 100℃. The absorbance was measured at 675nm after cooling for 30min,while galactose was used as the standard. The recovery of the procedure was 94.58%±1.68% and RSD was 0.548%(n=6).

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What this paper is about

The procedure of colorimetric analysis of TPS was investigated in this paper. The results showed that the accuracyof determination was largely improved by processing with the following procedure: 4ml of 0.05% anthrone in concentrated sulfuricacid was added to 1ml TPS solution and heated 5min at 100℃. The absorbance was measured at 675nm after cooling for 30min,while galactose was used as the standard. The recovery of the procedure was 94.58%±1.68% and RSD was 0.548%(n=6).

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Available abstract

The procedure of colorimetric analysis of TPS was investigated in this paper. The results showed that the accuracyof determination was largely improved by processing with the following procedure: 4ml of 0.05% anthrone in concentrated sulfuricacid was added to 1ml TPS solution and heated 5min at 100℃. The absorbance was measured at 675nm after cooling for 30min,while galactose was used as the standard. The recovery of the procedure was 94.58%±1.68% and RSD was 0.548%(n=6).

Key concepts: Anthrone, Chromatography, Sulfuric acid, Chemistry, Absorbance, Organic chemistry

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