Study on HPV genotype in cervical intraepithelial neoplasia

Objective:To explore the relationship between human papillomavirus(HPV) infection of different genotypes and the occurrence of cervical intraepithelial neoplasia(CIN) of different grades.Methods:284 patients diagnosed as CIN definitely in outpatient department of gynecology in the hospital from November 2007 to December 2009 were analyzed retrospectively;PCR amplification and gene chip probe hybridization were used to detect HPV genotypes.Results:The infection rate of HPV increased gradually with the increase of CIN grades.Among the patients with CIN Ⅰ,low risk HPV infection was the main type;among the patients with CIN Ⅲ,single high risk HPV-16 infection was the dominant type,followed by HPV-58.There was significant difference in infection rate of HPV-16 between CIN Ⅰ and CIN Ⅱ,as well as between CIN Ⅱ and CIN Ⅲ(P0.01).Conclusion:The infection rate of HPV increase gradually with the development of CIN,the genotypes of HPV infection are different,the main HPV genotypes of CIN Ⅰ are HPV-11 and HPV-6;the main HPV genotypes of CIN Ⅱ are HPV-16 and HPV-6;the main HPV genotypes of CIN Ⅲ are HPV-16 and HPV-58.The relationship between infection rate of HPV-16 and grades of CIN is from quantitative relationship to qualitative relationship.