Relative correction factor determination of 5 major compounds in the extract from Ixeris Sonchifolia Hance by HPLC

Objective To determine the relative correction factors(RCF) between luteolin-7-O-β-D-glucuronopyranoside and another 4 phenolic acids:caffeic acid,cichoric acid,luteolin-7-O-β-D-glucopyranoside(1→6)-β-D-glucopyranoside,and apigenin-7-O-β-D-glucuronopyranoside.All the 5 compounds in the extract from Ixeris Sonchifolia Hance were determined by only one reference substance.Methods The Agilent TC-C18 column(250 mm×4.6 mm,5 μm)was 30 ℃,the mobile phase was composed of methanol(A) and 0.05% phosphoric acid(B):0-10 min,30%A,10-30 min,30%-50%A.The flow rate was 1.2 mL·min-1,and the detection wavelength was 350 nm.Results The relative correction factors of caffeic acid,cichoric acid,luteolin-7-O-β-D-glucopyranoside(1→6)-β-D-glucopyranoside,and apigenin-7-O-β-D-glucuronopyranoside were identified as 1.129,0.873 3,1.320,and 1.129(RSD=1.6%-2.1%).The content of the 5 compounds were 4.2%,29.5%,6.5%,23.0%,and 5.0%.Conclusion The method is simple,accurate,sensitive and reproducible,which can be used in the quantitative analysis and quality control of the extract and preparation of Ixeris Sonchifolia Hance.