Experimental study of protection effects of non-myocardial ischemic preconditioning on immature myocardium and myocardial interstitium in neonatal rabbits

AIM:To determine the protection effects of non-myocardial ischemic precoditioning on immature myocardium and myocardial interstitium . METHODS:Isolated perfused neonatal rabbit hearts from group ischemic-reperfucion(I/R),group ischemic preconditioning(IPC),group double limb-ischemic preconditioning (DL-IPC) and group kidney-ischemic preconditioning(K-IPC) were underwent 45 minute ischemic followed 45 minute reperfusion before group I/R no IPC,group IPC with 2×IPC(5 minute ischemia followed 5 minute reperfusion),group DL-IPC with 3×IPC(double limbs were obstructed 5 minute followed 5 minute reperfusion). The K-IPC with 3×IPC(right kidney artery was clamped 5 minute followed 5 minute reperfusion). The left ventricular function recovery,MWC,LDH and CK leakage,MDA and ATP content and SOD activity HP and ET content were tested. RESULTS:The recovery of postischemic heart function,ATP content,SOD activity and HP content in group IPC,DL-IPC and K-IPC were higher than that of group I/R. MWC,MDA and ET content,LDH and CK leakage in group IPC,DL-IPC and K-IPC were lower than that of group I/R. CONSLUCION:DL-IPC and K-IPC had the same cardioprotection to immature myocardium and myocardial interstitium as IPC.