Experimental study of immature cardioprotection at different sites ischemic preconditioning in neonatal rabbits

Objective To determine the immature myocardial protection effects at different at sites ischemic preconditioning. Methods Isolated perfused neonatal rabbit hearts from group ischemic-reperfusion(I/R), group ischemic preconditioning(IPC), group double limb-ischemic preconditioning(DL-IPC) and group kidney-ischemic preconditioning(K-IPC) were underwent 45-minute ischemia followed 45-minute reperfusion before group I/R no IPC, group IPC with 2×IPC(5-minute ischemia followed 5-minute reperfusin), group DL-IPC with 3×IPC(double limbs obstructed 5-minute followed 5-minute reperfusion), group K-IPC with 3×IPC(right kidney artery clamped 5-minute followed 5-minute reperfusion), but group normal controll(NC) no ischemia only perfused KH solution 70-minute. The left ventricular function recovery, MWC, LDH and CK leakage, MDA and ATP content, SOD activity and myocardial ultrastructure were tested. Results The recovery of postischemic heart function, ATP content, SOD activity and myocardial ultrastructure in group IPC, DL-IPC and K-IPC were higher than that of group I/R. MWC, MDA content, LDH and CK leakage in group IPC, DL-IPC and K-IPC were lower than that of group I/R. Conclusion DL-IPC and K-IPC and the same cardioprotection to immature myocardium as IPC.