Simultaneous Determination of Contents of Triamcinolone Acetonide Acetate and Miconazole Nitrate in Triamcinolone Acetonide Acetate and Miconazole Nitrate and Neomycin Sulfate Cream by RP-HPLC

Objective To establish an RP-HPLC method for the simultaneous determination of contents of triamcinolone acetonide acetate and miconazole nitrate in triamcinolone acetonide acetate and miconazole nitrate and neomycin sulfate cream.Methods RP-HPLC was conducted using a Diamonsil C18 column(200 mm×4.6 mm,5 μm),with a mobile phase of 0.5% ammonium acetate solution--acetonitrile--methanol(15:42.5:42.5)and flow rate of 1.0 mL·min-1.Samples was detected at 272 nm,with dibutyl phthalate as internal standard.Results The linear range was 64.08-149.52 μg·mL-1(r=0.999 9)and the average recovery rate was 99.6%,RSD=0.43%(n=6)for triamcinolone acetonide acetate.The linear range was 600.6-1 401.4 μg·mL-1(r=0.999 9),the average recovery rate was 100.0%,RSD=0.49%(n=6)for miconazole nitrate.Conclusion The method was accurate,simple and effective.It might effectively be applied to control the quality of agent.