Purification technology of polyphenol from Korean pine cone lamella with macroporous resins and research of their antioxidant ability

According to adsorption and desorption property of macroporous resins to polyphenol,an optimal macroporous resin was chosen from ADS-7、S-8、NKA-9、NKA-Ⅱ、HPD600、AB-8、X-5、D101、D3520 resins for purifying polyphenol from Korean pine cone lamella.AB-8 was found to be the most appropriate resin.The purification of polyphenol was studied,optimum conditions were as follows:the loaded amount was 0.3BV,the concentration of polyphenol was 1.5mg/mL,the time static absorption was 3h,the volume of elution water was 3BV,the eluting solvent was 90% alcohol,the volume of alcohol was 1.6BV.Under these conditions,the purity of polyphenol was 12.51% in the purified samples,while it was 35.07% in the crude samples.In addition,the comparative study on the antioxidant ability was performed between crude and purified samples.The result showed that Fe3+ reducing power and the scavenging activity on DPPH radical were enhanced after the samples were purified.