SEPARATION AND PURIFICATION OF APPLE POLYPHENOL WITH THE MACROPOROUS RESINS

Taking the contents of total apple polyphenol in the gala apple as index, the separated and purified technology of apple polyphenol was studied by Macroporous Resins AB-8.As the result of the test shows, the purity of the apple polyphenol was effectively improved by Macroporous Resins AB-8.By the research of influence factors of purification, the optimum purification process of crude extracts of ethanol precipitation removing impurity were determined by Macroporous Resins AB-8, and the optimum solution of apple polyphenol was 1.5 g/L solution, the optimum sample injection rate was 1 mL/min, eluted to non-sugar by distilled water after adsorption saturation, and then 60 % acetone elution that the optimum elution speed was 0.5 mL/min, in the end, 79.5 % the purification of apple polyphenol was obtained.