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2009•Anhui Medical and Pharmaceutical JournalRequires access

Determination of guercetin in shengshitong granule by HPLC

Min Zhang

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Abstract

Aim To estalish a method for determination of the content of quercetin in shenshitong granule.Methods HPLC method was used.The sample was separated by Kromasil C18 column(250 mm×4.6 mm,5 μm).The mobile phase was methanol-0.4% phosphoric acid(50∶50).The detection wavelength was 370 nm.Flow rate was 1.0 ml·min-1.the column temperature was at 25℃.Results The linear range of quercetin is between 5.20 mg·L-1 and 20.80 mg·L-1.The average recovery is 98.9%(RSD=0.40%,n=9).Conclusions The method is sensitive,rapid and accurate.It is suitable for determining shenshitong grainule.

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What this paper is about

Aim To estalish a method for determination of the content of quercetin in shenshitong granule.Methods HPLC method was used.The sample was separated by Kromasil C18 column(250 mm×4.6 mm,5 μm).The mobile phase was methanol-0.4% phosphoric acid(50∶50).The detection wavelength was 370 nm.Flow rate was 1.0 ml·min-1.the column temperature was at 25℃.Results The linear range of quercetin is between 5.20 mg·L-1 and 20.80 mg·L-1.The average recovery is 98.9%(RSD=0.40%,n=9).Conclusions The method is sensitive,rapid and accurate.It is suitable for determining shenshitong grainule.

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Available abstract

Aim To estalish a method for determination of the content of quercetin in shenshitong granule.Methods HPLC method was used.The sample was separated by Kromasil C18 column(250 mm×4.6 mm,5 μm).The mobile phase was methanol-0.4% phosphoric acid(50∶50).The detection wavelength was 370 nm.Flow rate was 1.0 ml·min-1.the column temperature was at 25℃.Results The linear range of quercetin is between 5.20 mg·L-1 and 20.80 mg·L-1.The average recovery is 98.9%(RSD=0.40%,n=9).Conclusions The method is sensitive,rapid and accurate.It is suitable for determining shenshitong grainule.

Key concepts: Chromatography, Phosphoric acid, Granule (geology), High-performance liquid chromatography, Chemistry, Linear range, Quercetin, Analytical Chemistry (journal)

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