Tynamic changes of MMP-2 and MMP-9 and the effect of sodium aescinate in rats with intracerebral hemorrhag

Objective To observe relationship the dynamic expression of matrix metalloproteinase-2(MMP-2)and MMP-9 protein with water contents in perihematoma,and the effect of sodium aescinate in rats with intracerebral hemorrhage(ICH).Methods 250 Wistar rats were assigend randomly to four groups:normal control group(10 rats),sham operation group(80 rats),ICH group(80 rats)and sodium aescinate treating group(80 rats).the water contents in perihematom and the expressions of MMP-2 and MMP-9 protein were measured respectively at 6、12、24、48、72、120h、7d and 15d after operation in every group.Results Neurological function deficit in the treating gruoup was improved than the ICH group(P0.05).The water contents in perihematoma in the treating group were reduced than the ICH group at 6～120h(P0.05).The expression of MMP-2 protein peaked at 6h,and decreased at 12h,and were compared with the normal control group,it had statistic significance obviously(P0.01)after ICH.The expression of MMP-2 protein in the treating group was reduced than the ICH group at various time stages(P0.01).The expression of MMP-9 protein increased at 6h,peaked at 24～48h,and decreased at 72h,and was compared with the normal control guoup,it had statistic significance obviously(P0.01),the changes of the contents of MMP-9 protein were positively correlated with water contents in perihematoma(r=0.949,P0.05)after ICH.the expression of MMP-9 protein in the treating group was reduced than the ICH group at different time point(P0.01). Conclusion The expression of MMP-2 and MMP-9 protein play the important roles in the brain edema at early and middle period after ICH.Sodium aescinate may reduce the expression of MMP-2 and MMP-9 protein and decreased the water contents in perihematoma.The drug may have protective effect to ICH.