2002•Integrated Traditional Chinese and Western Medicine In Practice of Critical Care MedicineRequires access
Protective effects of puerarin injection on renal ischemia-reperfusion injury in rats
Rongguo Fu
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Abstract
Objective:To investigate the role of puerarin on acute renal ischemiareperfusion injury and its mechanism.Methods:Acute renal ischemiareperfusion iniury rat model was established by placing the atraumatic vascular clamp in renal pedical for 30 minutes.Rats in puerarin group were given puerarin injection 30 mg/kg before ischemia.Malondialdehyde (MDA) assay for lipid peroxides in rats was detected by thiobarbituric acid reaction,superoxide dismutase (SOD) activity was detected by subnitrate method,and thromboxane B 2(TXB 2) and 6ketoprostaglandin F 1α (6ketoPGF 1α ) were detected by radioimmunoassay.Results:After renal ischemiareperfusion injury in rats,the level of MDA was lower and the activity of SOD was higher in puerarin group than those in the control.Much less necrotic tubular cells were found in puerarin group.At the same time,the level of TXB 2 increased significantly,but 6ketoPGF 1α didn't increase significantly after reperfusion 18 hours in puerarin group than those in the control.There was no difference of the vital signs change between 2 groups.Conclusions:Chinese herb puerarin can protect kidney against ischemia after acute renal ischemiareperfusion injury which may associate with the action of against oxidation and change the balance of plasma TXA 2PGI 2 in rats.