Resistance Induced by β-aminobutyric Acid in Tobacco to Tobacco Mosaic Virus
Fang Ding
Abstract
Fang Ding
Abstract
[Objective] The study aimed to research the effect of β-Aminobutyric acid(BABA) on the resistance in tobacco to the systemic infection of tobacco mosaic virus(TMV).[Method] With the common cultivated species of tobacco as the researched object,it was treated by BABA.All the tobacco seedlings were put into the moisture retention bag to keep moisture for 24 h after treatment.Then the allergic reaction of inducing tobacco generation by BABA with histochemistry method,the generation H2O2 in suit was measured and reactive oxygen species and malondialdehyde were measured.[Result] The activity of β-1,3-glucanase in leaves was improved remarkably after treatment by BABA,which was also improved in leaves after TMV inoculation.The sprinkle treatment of 10 mmol/L BABA could obviously induce oxygen burst,cause hypersensitive response,make foliar local cellular necrosis.The content of superoxide anion radicals and malondialdehyde in tobacco leaves was increased after BABA treatment.10 mmol/L BABA could effective induce systemic acquired resistance,prevent tobacco from TMV invading and enhance resistance to TMV in tobacco plants.[Conclusion] BABA could improve the ability of the common cultivated species of tobacco on resistance TMV systemic infection,but its induce resistance ability was influenced by its concn..
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[Objective] The study aimed to research the effect of β-Aminobutyric acid(BABA) on the resistance in tobacco to the systemic infection of tobacco mosaic virus(TMV).[Method] With the common cultivated species of tobacco as the researched object,it was treated by BABA.All the tobacco seedlings were put into the moisture retention bag to keep moisture for 24 h after treatment.Then the allergic reaction of inducing tobacco generation by BABA with histochemistry method,the generation H2O2 in suit was measured and reactive oxygen species and malondialdehyde were measured.[Result] The activity of β-1,3-glucanase in leaves was improved remarkably after treatment by BABA,which was also improved in leaves after TMV inoculation.The sprinkle treatment of 10 mmol/L BABA could obviously induce oxygen burst,cause hypersensitive response,make foliar local cellular necrosis.The content of superoxide anion radicals and malondialdehyde in tobacco leaves was increased after BABA treatment.10 mmol/L BABA could effective induce systemic acquired resistance,prevent tobacco from TMV invading and enhance resistance to TMV in tobacco plants.[Conclusion] BABA could improve the ability of the common cultivated species of tobacco on resistance TMV systemic infection,but its induce resistance ability was influenced by its concn..
Key concepts: Tobacco mosaic virus, Malondialdehyde, Inoculation, Hypersensitive response, Superoxide, Reactive oxygen species, Tobamovirus, Horticulture