Study on the Protection of Tobacco by β-Aminobutyric Acid against Infection of Tobacco Mosaic Virus

[Objective] The aim was to study the protection of tobacco by β-aminobutyric acid,to enhance the anti-TMV infection ability.[Method] With the common cultivated species of Yunyan 87 as the researched object,and it was sprinkled by 0.5,1.0,5.0,7.0 and 10.0 mmol/L BABA respectively,water-treated as control,counted the rate of inhibition of BABA to TMV and screened the most appropriate BABA treatment concentration.After 5.0 mmol/L BABA treatment,phenotypic disease state of seedlings in the growth process was observed,the allergic reaction of inducing tobacco generated by BABA was measured with histochemistry method,the generation of H_2O_2 was measured by DAB histochemical position staining and the activity of PAL was measured.Pathogenesis related(PR) proteins were measured by SDS-PAGE.[Result] The treatment of BABA could obviously induce oxygen burst,cause hypersensitive response.The activity of PAL in leaves was improved remarkably after treatment by BABA,a possible PR protein,which molecular weight 20 kDa was found.And the morbidity of TMV was reduced obviously,thus,BABA enhanced TMV resistance of tobacco.[Conclusion] The study provided theoretical basis for further revealing the induction mechanism of BABA.