Effect of xuebijing injection on NF-κB expression of kidney after ischemia-reperfusion of limbs in rats

Objective To study the changes of NF-κB expression in kidney tissues and the protective effect of xuebijing injection on ischemic-reperfusion (I/R) of hind leg of rats. Methods The I/R models were prepared on thirty Wistar male rats,and were divided into five groups: A,control group;B,ischemia only group; C,ischemia- reperfusion group; D,I/R with low dosage xuebijing injection group;E,I/R with high dosage xuebijing injection group. Serum level of CK and content of MDA in kidney tissues of each group were examined.At the same time,the expression of NF-κB in kidney tissue was detected with real-time polymerase chain reaction and immunohistochemical technique,respectively. Results Compared with control group,the expression level of NF-κB increased in ischemia group and significantly increased in I/R group(P0.01); in two xuebijing treated groups,the expression level of NF-κB decreased,but there was no difference between groups D and E(P0.05). Compared with control group,serum level of CK and kidney tissues content of MDA were increased in ischemia group and I/R group(P0.01). Compared with I/R group,serum level of CK and kidney tissues content of MDA were decreased in xuebijing treated group(P0.01). Conclusions The expression of NF-κB in kidney tissue is significantly upregulated in limbs of I/R model rats. The xuebijing injection has protective effect on kidney injury against skeletal musle ischemia-reperfusion injury and the protective effect is not dosage related.