Protective effect of Xuebijing injection on kidney injury after hepatic ischemia-reperfusion in rats

Objective To investigate the protective role and mechanismof Xuebijing injection in kidney ischemia-reperfusion injury in rats.MethodsNinety male Wistar rats were randomly divided into sham operation control group(SOC group),ischemia-reperfusion group(I/R group) and Xuebijing group (XBJ group),30 animals in each group.Xuebijing injection (5 ml·kg-1·d-1) was administered to animals in XBJ group for three days before operation.Six hour,twelve hour,twenty hour after reperfusion,venous blood samples and kidney tissue samples were taken for measurement. 10 rats were randomly chosen in each group.The levels of serum creatinine (Cr),Malon dialdehyde (MDA) and activity of superoxide dismutase (SOD) in kidney tissue were determined. The kidney tissue was examined under light microscopy.ResultsAfter reperfusion,serum levels of Cr and levels of MDA in kidney tissue of XBJ group were significantly higher than those in I/R group(P0.01).The histological changes of the kidney tissue under light microscopy in I/R group was more obvious than that in XBJ group.ConclusionXuebijing injection can protect kidney from ischemia-reperfusion injury.The mechanism of the protective effect of Xuebijing injection may be related with the decrease of oxygen free radical.