Synergistic effect of magnetic nanopartical Fe_3O_4 with cisplatin on pulmonary adenocarcinoma cell A549

Objective To study the effect of magnetic nanopartical Fe3O4 with cisplatin on the expression level of apoptosis-related genes and drug resistance protein in pulmonary adenocarcinoma cell A549 and to explore the different mechanisms in increasing the therapeutic effectiveness.Methods The inhibitory effects of magnetic Nano-Fe3O4 and cisplatin on the proliferation A549 cell line were evaluated by MTT assay.Cell apoptosis was detected by flow cytometry.The changes of bcl-2,survivin and ERCC1 mRNA were quantified by RT-PCR.The expressive rates of multi-drug resistant-associated protein MRP were quantified by Western blotting.Results 25 μg·ml-1Nano-Fe3O4 with cisplatin induced more apoptosis percentage.Compared to the control,Nano-Fe3O4 or DDP treatment group,there existed a decrease in bcl-2 and ERCC1 mRNA expression in the combination group,while survivin remained unchanged.And a decrease in the expression of multidrug resistance associated protein MRP was observed.Conclusion The Nano-Fe3O4 with cisplatin significantly increases the therapeutic effectiveness in A549 cells by inducing more apoptosis and decreasing the expression of bcl-2,ERCC1 mRNA and MRP.