Changes and significances of apoptosis and autophagy levels of ovarian cancer sensitive cells-A2780 and resistant cells-A2780/DDP after treated with cisplatin

Objective:To explore the relationship between autophagy and drug resistance to cisplatin in patients with ovarian cancer by analyzing the effect of autophagy on sensitivities of ovarian cancer cells to cisplatin,provide a basis for the treatment of ovarian cancer.Methods:Ovarian cancer cell strains-A2780 and A2780/DDP were cultured,then their sensitivities to cisplatin were detected,flow cytometry was used to detect the apoptosis rates of A2780 cells and A2780/DDP cells after treated with cisplatin;Western blot was used to detect the expression levels of Beclin 1 protein in A2780 cells treated with 10 μM cisplatin for 48 hours and A2780/DDP cells treated with 40μM cisplatin for 48 hours;A2780 cells and A2780/DDP cells were fixed and stained,and the activity of autophagocytes were observed under electron microscope.Results:Cisplatin played cytotoxic effect on ovarian cancer sensitive cells-A2780 and resistant cells-A2780/DDP by inducing apoptosis;after treated with cisplatin,the autophagy activity of sensitive cells increased significantly,while the autophagy activity of resistant cells did not increase obviously.Conclusion:The ability of cisplatin inducing the apoptosis of ovarian cancer resistant cells is lower than that of ovarian cancer parent sensitive cells;the increase of reactive autophagy activity in the course is related to the formation of cisplatin resistance,which indicates that monitoring and regulating the autophagy levels of tumor cells may provide a new idea for reversing cisplatin resistance in ovarian cancer.