Isolation and Degradation Characteristics of Nitrobenzene-degradation Bacterium at Low Temperature

6 strains with nitrobenzene as sole carbon source at low temperature were isolated from activated sludge of aeration tank and Chloramphenicol production wastewater collection tank in Northeast Pharmaceutical Plant.The study of biodegradation capability showed that cc-2 was the effective nitrobenzene-degrading strain.According to the morphological characteristics,physiological and biochemical characteristics,the strain was identified as Acinetobacter sp.Results of degradation characteristics showed that under the conditions of the optimal temperature 15 ℃,pH 7 and shaker rotary speed 140 r/min,the degradation rate was 66.84% in 48 h when the initial nitrobenzene concentration was 200 mg/L.The additional glucose and sodium acetate accelerated the biodegradation of nitrobenzene at low temperature,with the nitrobenzene degradation rate up to 80.44% and 78.57% respectively.The study of growth and degradation characteristics would provide technical support for the treatment of nitrobenzene.