Isolation and identification of a nitrobenzene-degrading strain at low temperature

A strain that can utilize nitrobenzene as sole source of carbon at low temperature was isolated from activated sludge of the aeration tank and the chloramphenicol production wastewater collection pond in a northeast pharmaceutical factory.The strain,named cc-2,has a high degradation rate for nitrobenzene.According to its morphological characteristics、physiological and biochemical characteristics and 16S rDNA,strain cc-2 was identified as Acinetobacter baumannii.Study on its growth and degradation characteristics showed that the optimal inoculum was 10% 、temperature 15℃ and pH 7 for strain cc-2 utilizing nitrobenzene.Strain cc-2 can grow in medium with less than 400 mg.L-1 of nitrobenzene.The degradation rate of cc-2 in 48 h was 66.84% when the initial concentration of nitrobenzene was 200 mg.L-1.