Expression of CK19 and its significance in breast cancer

Objective:To evaluate the clinical application of fluorescent quantification polymerase chain reaction(FQ-PCR) to detect CK19 in the breast cancer.Methods: CK19 in 30 health women,30 patients with benign breast disease and 91 patients with breast cancer were detected by FQ-PCR.β2-microglobin was used as internal control.Results: There were no significant difference in CK19/β2-microglobin between normal controls and benign breast disease group(P0.05).CK19/β2-microglobin in breast cancer group were higher than those in the other groups(P0.05).There were no difference in β2-microglobin among three groups(P0.05).The positive ratio of breast cancer and benign disease were 49.5 and 0 percent respectively.No significant association of CK19 expression among age and tumor size and type was noted(P0.05),but its expression in breast cancer was correlated with clinical stage,histological grade and axillary metastatic lymph node(P0.05).Conclusion: FQ-PCR is a rapid and sensitive and specific method for quantitating CK19.It also gives objective evidence to the diagnosis,therapy,metastasis of breast cancer.