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Studies on UV Induced Screening of Streptomyces 702 Under Lincomycin-resistant Lethal Dose

TU Guo-quan

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Abstract

The paper applied UV and lincomycin-resistant method to screen high-yield mutants of Streptomyces 702.The results showed that the lincomycin-resistant mutational frequency was 20.77% after the spores of Streptomyces 702 being treated with UV 20 seconds.The high-yield mutants were screened at a high frequency(25%).Furthermore,the high-yield mutational frequency was 10%,which theirs productivities were 20% higher than the original strain.The mutant 42-3473 production was up to 2 062 μg/mL,which was 70% higher than that of the original strain.

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What this paper is about

The paper applied UV and lincomycin-resistant method to screen high-yield mutants of Streptomyces 702.The results showed that the lincomycin-resistant mutational frequency was 20.77% after the spores of Streptomyces 702 being treated with UV 20 seconds.The high-yield mutants were screened at a high frequency(25%).Furthermore,the high-yield mutational frequency was 10%,which theirs productivities were 20% higher than the original strain.The mutant 42-3473 production was up to 2 062 μg/mL,which was 70% higher than that of the original strain.

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Available abstract

The paper applied UV and lincomycin-resistant method to screen high-yield mutants of Streptomyces 702.The results showed that the lincomycin-resistant mutational frequency was 20.77% after the spores of Streptomyces 702 being treated with UV 20 seconds.The high-yield mutants were screened at a high frequency(25%).Furthermore,the high-yield mutational frequency was 10%,which theirs productivities were 20% higher than the original strain.The mutant 42-3473 production was up to 2 062 μg/mL,which was 70% higher than that of the original strain.

Key concepts: Lincomycin, Streptomyces, Yield (engineering), Mutant, Strain (injury), Spore, Microbiology, Biology

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