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2009•Unpublished venueRequires access

Analysis of Antibiotic Resistance of Metallo-β-lactamase of Pseudomonas Aeruginosa in Clinical Isolates

WU Jua

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Abstract

Objective To analyze the detection method and the resistance profile of metallo-β-lactamase Pseudomonas aeruginosa in clinical isolates.Methods A total of 40 P.aeruginosa isolates in our hospital were analyzed.The phenotype of Metallo-β-lactamase were screened by EDTA-IMP disk diffusion method and E-test method and PCR method.Susceptibility test was performed by determing minimum inhibitory concentrations.Results Nine of 40 imipenem-resistant Pseudomonas aeruginose isolate by EDTA-IMP disk diffusion method,6 of the strains were screened by E-test method,1 of the strains was found to produce IMP-1 and 3 of the strains were found to produce VIM-2.The strains produced Metallo-β-lactamase were multi-drug resistance,the strains with positive in PCR method highly resistant.The susceptibility to amikacin and ciprofloxacin highly active.Conclusion Isolates of multi-drug resistance pose a serious situation of Metallo-β-lactamase of Pseudomonas aeruginosa in patients.

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Objective To analyze the detection method and the resistance profile of metallo-β-lactamase Pseudomonas aeruginosa in clinical isolates.Methods A total of 40 P.aeruginosa isolates in our hospital were analyzed.The phenotype of Metallo-β-lactamase were screened by EDTA-IMP disk diffusion method and E-test method and PCR method.Susceptibility test was performed by determing minimum inhibitory concentrations.Results Nine of 40 imipenem-resistant Pseudomonas aeruginose isolate by EDTA-IMP disk diffusion method,6 of the strains were screened by E-test method,1 of the strains was found to produce IMP-1 and 3 of the strains were found to produce VIM-2.The strains produced Metallo-β-lactamase were multi-drug resistance,the strains with positive in PCR method highly resistant.The susceptibility to amikacin and ciprofloxacin highly active.Conclusion Isolates of multi-drug resistance pose a serious situation of Metallo-β-lactamase of Pseudomonas aeruginosa in patients.

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Available abstract

Objective To analyze the detection method and the resistance profile of metallo-β-lactamase Pseudomonas aeruginosa in clinical isolates.Methods A total of 40 P.aeruginosa isolates in our hospital were analyzed.The phenotype of Metallo-β-lactamase were screened by EDTA-IMP disk diffusion method and E-test method and PCR method.Susceptibility test was performed by determing minimum inhibitory concentrations.Results Nine of 40 imipenem-resistant Pseudomonas aeruginose isolate by EDTA-IMP disk diffusion method,6 of the strains were screened by E-test method,1 of the strains was found to produce IMP-1 and 3 of the strains were found to produce VIM-2.The strains produced Metallo-β-lactamase were multi-drug resistance,the strains with positive in PCR method highly resistant.The susceptibility to amikacin and ciprofloxacin highly active.Conclusion Isolates of multi-drug resistance pose a serious situation of Metallo-β-lactamase of Pseudomonas aeruginosa in patients.

Key concepts: Amikacin, Pseudomonas aeruginosa, Imipenem, Microbiology, Ciprofloxacin, Agar diffusion test, Antibiotics, Drug resistance

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