Protective effects and mechanism of effective components group of Xiaoshuantongluo on cerebral ischemia injury in rats

Objective To observe the protective effect and mechanism of effective components group of Xiaoshuantongluo(XECG)on ischemia/reperfusion(I/R)injury in rats.Methods Healthy male adult Sprague Dawley rats were randomly divided into the sham operation group,the model group,the nimodipine(4mg·kg-1)group,and high,middle and low dose XECG groups(200,100,50mg·kg-1).Middle cerebral artery occlusion was adopted to establish the rat ischemia/reperfusion model.After ischemia for 120 min and reperfusion for 24 h,the pathological injury of the ischemia side was observed by HE staining.Expressions of tumor necrosis factorα(TNF-α),Interleukin 1β(IL-1β)were detected by western blotting technique.Results XECG could significantly improve the pathological changes of cortical penumbra brain tissues,Compared with the I/R model group,XECG could significantly inhibit expressions of TNF-α、IL-1β.Conclusion XECG has obviously protective effect on Sprague Dawley rats after cerebral ischemia and reperfusion,its mechanisms may be associated with inhibiting inflammatory,expression of TNF-αand IL-1βproteins.