Several Physiological Factors Influencing Adventitious Bud Regeneration from the Epicotyls of Ponkan (Citrus reticulata Blanco)

Several physiological factors influencing the bud regeneration from the epicotyls of Ponkan (Citrus reticulata Blanco) were studied. The results showed that epicotyl segments horizontally placed in the medium and 20-d dark incubation followed by the continuous light condition enhanced the bud regeneration from the Ponkan epicotyl segments. However, the best growth quality of the adventitious buds was obtained when epicotyl segments inserted in the medium with the morphological lower end. The highest rate of adventitious bud regeneration was reached when the MT medium supplied with 6-BA 1 mg·L-1 and NAA 0.1 mg·L-1. The plantlet formed when the buds were subcultured in 1/2MT with 0.1-1 mg·L-1 NAA.