The Expression of Fas/FasL and its mechanism of immune escape in the choriocarcinoma cell lines

Objective To observe the expression of Fas / FasL in choriocarcinoma cell lines (JEG-3 and BeWo),its apoptosis and immune escape mechanism. Methods RT-PCR method was used to detect the mRNA expression of Fas / FasL in JEG-3,BeWo and Jurkat cell lines,Western blot assay was used to detect Fas / FasL protein expression. Trypan blue dye assay was used to detect the survival rate of Jurkat cells. Flow cytometry (FCM) was used to monitor the cell apoptosis. Results Compared with Jurkat cells,the expression of Fas mRNA was lower ,while FasL mRNA was higher in choriocarcinoma cell lines with a significant difference.Co-cultured with Jurkat cells ,choriocarcinoma cells could induce Jurkat cell apoptosis (P0.05),with extended culture time,the apoptosis rate of Jurkat cells was notably increased(P0.05).Conclusion The choriocarcinoma cell can resist the apoptosis induced by Fas / FasL system,the higher expression of FasL can inhibit immune function,and the lower Fas expression in T cells can evade immune destruction.