A study on epithelium tumor vaccine in urologic system

Objective To identify and characterize the mimic epitope of MUC1 from phage display random 12 peptide library.Methods The purified Ma695 antibody was used to screen in phage display random 12 peptide library.The positive clones were identified by sandwich ELISA,competitive inhibition assay.The homologous comparison of amino acid sequences of positive clones was conducted through searching the protein sequence databank.The digestion analyses of amino acid sequences were also conducted by software.Results 14 positive clones were acquired after 3 rounds of screening.Amino acid sequences deduced from DNA sequences showed four different sequences as KHYDPFHHRMPQ,QADTARSVALAG,VPSKPDLHVRSI,MTPIHYWNHNRV.The inhibitory assay showed that the 4 mimic epitope peptides displaying on the phage surface could effectively inhibit the combination of antibody with antigen and the inhibitory rates of each mimic epitope were 50% higher than that in the control.Conclusion The results shows that KHYDPFHHRMPQ,QADTARSVALAG,VPSKPDLHVRSI,MTPIHYWNHNRV are the mimotopes which could mimic the epitope of MUC1.