2000•Di-Si Junyi Daxue xuebaoRequires access
Selection of mimic epitope of gastric cancer associated antigen by using phage peptide library
Han Quan
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Abstract
AIM To select mimic epitope of gastric cancer associated antigen by using phage display peptide library. METHODS ①Anti gastric cancer monoclonal antibody MG 7 Ab was purified from mouse ascites fluid using ion exchange chromatography method. ②Biopanning of phage display peptide library. By using the MG 7 Ab as selective molecular, a 7 meres phage display peptide library was biopanned, and the positive clones were selected. ③Identification of the epitope: the positive clones were confirmed by DNA sequencing, amino acid sequence assay. RESULTS Twelve positive clones were chosen and a candidate epitope (KPHxHxH) was identified by amino acid sequence assay. CONCLUSION A candidate mimic epitope of gastric cancer associated antigen can be selected, thus providing the potential for developing tumor peptide vaccine.