2010•Tianjin yiyaoRequires access
Effect of Anti-Oxidant on Cytokines of Acute Lung Injury in Rats
Wei Liu, Hui Zhang, Cheng Chang, Shuxiang Lin, Jinsheng Zhao, Jie Zheng, Jing-fu Huang
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Abstract
Objective:To investigate the pathogenesis of acute lung injury (ALI), and the effect of anti-oxidants on cytokines of ALI in rats. Methods:Sixty Wistar rats were randomly divided into 2 groups, the model group and the treatment group. The animal model of ALI was established by intraperitoneal injection of Escherichia(E.)coli. The anti-oxidant, reduced glutathione, was injected by the rat tail vein 30 min after injection of E. coli in rats of treatment group. The levels of serum IL-8 and IL-10 were detected 3, 6 and 12 h after injection of E. coli. Results:The symptom of ALI appeared 3 h after injection of E. coli, and was more serious after 6 h in rats of the model group. There were anatomical lung edema and bleeding points 12 h after injection of E. coli in rats of the model group. The oxygenation index and lung wet/dry weight ratio reached the diagnostic criteria of ALI. There was no significant change after 3 h, rapid breath after 6 h and only mild lung edema after 12 h in rats of the treatment group. The value of lung wet/dry weight ratio was lower in rats of treatment group than that of model group, which was significantly different after 6 and 12 h treatment (P 0.05). The levels of IL-8 w ere lower at various time points in treatment group than those of model group(P 0.05). The levels of IL-10 were significantly higher at different detection time points in treatment group than those of model group (P 0.05). Conclusion:There was an imbalance and uncontrol of inflammatory mediators in acute lung injury. After application of reduced glutathione, inflammatory mediators can be effectively controlled, thereby reducing the occurrence probability of the acute lung injury.